aurora addgene 98217 Search Results


addgene plasmid repository  (Addgene inc)
90
Addgene inc addgene plasmid repository
Addgene Plasmid Repository, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mcerulean3  (Addgene inc)
93
Addgene inc mcerulean3
(A) Molecular scheme of BiPOLES with the extracellular (EC) and intracellular (IC) ionic conditions used for HEK293-cell recordings. The blue-green-light-activated natural anion channels Gt ACR1 and Gt ACR2 or the engineered ChR-chimeras iC++ and Aurora were fused to the red-light-activated cation-conducting Chrimson by a transmembrane spanning linker region consisting of a trafficking signal (ts), a yellow or cyan fluorescent protein (EYFP, <t>mCerulean3)</t> and the βHK transmembrane fragment. The fusion construct termed BiPOLES is indicated by a black frame. (B) Representative photocurrents of βHK-Chrimson-mCerulean (top), Gt ACR1-ts-mCerulean-βHK-Chrimson (middle) Gt ACR2-ts-mCerulean-βHK-Chrimson (BiPOLES, bottom) in whole-cell patch clamp recordings from HEK293 cells at 490, 600 and 650 nm illumination. (C) Normalized peak photocurrents of BiPOLES at different membrane voltages evoked at either 490 or 600 nm (see panel B, mean ± SD; n = 6 - 8; normalized to the peak photocurrent at −80 mV and 600 nm illumination). (D) Reversal potential of early peak photocurrents during 500-ms illumination with 490, 600, or 650 nm light as shown in (B) (mean ± SD; n = 5 - 8). (E) Top: Representative photocurrents of BiPOLES with 10 ms light pulses of different color and equal photon flux at −60 mV. Lower left: Action spectra of BiPOLES at different membrane voltages (λ rev = photocurrent reversal wavelength, mean ± SEM, n = 4 - 9). Lower right: λ rev of Gt ACR1-ts-mCerulean-βHK-Chrimson and BiPOLES at different membrane voltages (mean ± SD; n = 3 - 9). (F) Peak photocurrent densities at −80 mV and 600 nm or 650 nm illumination as shown in (B) (Mean ± SD; n = 5 - 8).
Mcerulean3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aurora+addgene+98217/bio_rxiv__2020__07__15__204347-77-38-17?v=Addgene+inc
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mcerulean3 - by Bioz Stars, 2026-08
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chlamydomonas noctigama  (Addgene inc)
91
Addgene inc chlamydomonas noctigama
(A) Molecular scheme of BiPOLES with the extracellular (EC) and intracellular (IC) ionic conditions used for HEK293-cell recordings. The blue-green-light-activated natural anion channels Gt ACR1 and Gt ACR2 or the engineered ChR-chimeras iC++ and Aurora were fused to the red-light-activated cation-conducting Chrimson by a transmembrane spanning linker region consisting of a trafficking signal (ts), a yellow or cyan fluorescent protein (EYFP, <t>mCerulean3)</t> and the βHK transmembrane fragment. The fusion construct termed BiPOLES is indicated by a black frame. (B) Representative photocurrents of βHK-Chrimson-mCerulean (top), Gt ACR1-ts-mCerulean-βHK-Chrimson (middle) Gt ACR2-ts-mCerulean-βHK-Chrimson (BiPOLES, bottom) in whole-cell patch clamp recordings from HEK293 cells at 490, 600 and 650 nm illumination. (C) Normalized peak photocurrents of BiPOLES at different membrane voltages evoked at either 490 or 600 nm (see panel B, mean ± SD; n = 6 - 8; normalized to the peak photocurrent at −80 mV and 600 nm illumination). (D) Reversal potential of early peak photocurrents during 500-ms illumination with 490, 600, or 650 nm light as shown in (B) (mean ± SD; n = 5 - 8). (E) Top: Representative photocurrents of BiPOLES with 10 ms light pulses of different color and equal photon flux at −60 mV. Lower left: Action spectra of BiPOLES at different membrane voltages (λ rev = photocurrent reversal wavelength, mean ± SEM, n = 4 - 9). Lower right: λ rev of Gt ACR1-ts-mCerulean-βHK-Chrimson and BiPOLES at different membrane voltages (mean ± SD; n = 3 - 9). (F) Peak photocurrent densities at −80 mV and 600 nm or 650 nm illumination as shown in (B) (Mean ± SD; n = 5 - 8).
Chlamydomonas Noctigama, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tiarina fusus  (Addgene inc)
93
Addgene inc tiarina fusus
(A) Molecular scheme of BiPOLES with the extracellular (EC) and intracellular (IC) ionic conditions used for HEK293-cell recordings. The blue-green-light-activated natural anion channels Gt ACR1 and Gt ACR2 or the engineered ChR-chimeras iC++ and Aurora were fused to the red-light-activated cation-conducting Chrimson by a transmembrane spanning linker region consisting of a trafficking signal (ts), a yellow or cyan fluorescent protein (EYFP, <t>mCerulean3)</t> and the βHK transmembrane fragment. The fusion construct termed BiPOLES is indicated by a black frame. (B) Representative photocurrents of βHK-Chrimson-mCerulean (top), Gt ACR1-ts-mCerulean-βHK-Chrimson (middle) Gt ACR2-ts-mCerulean-βHK-Chrimson (BiPOLES, bottom) in whole-cell patch clamp recordings from HEK293 cells at 490, 600 and 650 nm illumination. (C) Normalized peak photocurrents of BiPOLES at different membrane voltages evoked at either 490 or 600 nm (see panel B, mean ± SD; n = 6 - 8; normalized to the peak photocurrent at −80 mV and 600 nm illumination). (D) Reversal potential of early peak photocurrents during 500-ms illumination with 490, 600, or 650 nm light as shown in (B) (mean ± SD; n = 5 - 8). (E) Top: Representative photocurrents of BiPOLES with 10 ms light pulses of different color and equal photon flux at −60 mV. Lower left: Action spectra of BiPOLES at different membrane voltages (λ rev = photocurrent reversal wavelength, mean ± SEM, n = 4 - 9). Lower right: λ rev of Gt ACR1-ts-mCerulean-βHK-Chrimson and BiPOLES at different membrane voltages (mean ± SD; n = 3 - 9). (F) Peak photocurrent densities at −80 mV and 600 nm or 650 nm illumination as shown in (B) (Mean ± SD; n = 5 - 8).
Tiarina Fusus, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Molecular scheme of BiPOLES with the extracellular (EC) and intracellular (IC) ionic conditions used for HEK293-cell recordings. The blue-green-light-activated natural anion channels Gt ACR1 and Gt ACR2 or the engineered ChR-chimeras iC++ and Aurora were fused to the red-light-activated cation-conducting Chrimson by a transmembrane spanning linker region consisting of a trafficking signal (ts), a yellow or cyan fluorescent protein (EYFP, mCerulean3) and the βHK transmembrane fragment. The fusion construct termed BiPOLES is indicated by a black frame. (B) Representative photocurrents of βHK-Chrimson-mCerulean (top), Gt ACR1-ts-mCerulean-βHK-Chrimson (middle) Gt ACR2-ts-mCerulean-βHK-Chrimson (BiPOLES, bottom) in whole-cell patch clamp recordings from HEK293 cells at 490, 600 and 650 nm illumination. (C) Normalized peak photocurrents of BiPOLES at different membrane voltages evoked at either 490 or 600 nm (see panel B, mean ± SD; n = 6 - 8; normalized to the peak photocurrent at −80 mV and 600 nm illumination). (D) Reversal potential of early peak photocurrents during 500-ms illumination with 490, 600, or 650 nm light as shown in (B) (mean ± SD; n = 5 - 8). (E) Top: Representative photocurrents of BiPOLES with 10 ms light pulses of different color and equal photon flux at −60 mV. Lower left: Action spectra of BiPOLES at different membrane voltages (λ rev = photocurrent reversal wavelength, mean ± SEM, n = 4 - 9). Lower right: λ rev of Gt ACR1-ts-mCerulean-βHK-Chrimson and BiPOLES at different membrane voltages (mean ± SD; n = 3 - 9). (F) Peak photocurrent densities at −80 mV and 600 nm or 650 nm illumination as shown in (B) (Mean ± SD; n = 5 - 8).

Journal: bioRxiv

Article Title: BiPOLES: a tool for bidirectional dual-color optogenetic control of neurons

doi: 10.1101/2020.07.15.204347

Figure Lengend Snippet: (A) Molecular scheme of BiPOLES with the extracellular (EC) and intracellular (IC) ionic conditions used for HEK293-cell recordings. The blue-green-light-activated natural anion channels Gt ACR1 and Gt ACR2 or the engineered ChR-chimeras iC++ and Aurora were fused to the red-light-activated cation-conducting Chrimson by a transmembrane spanning linker region consisting of a trafficking signal (ts), a yellow or cyan fluorescent protein (EYFP, mCerulean3) and the βHK transmembrane fragment. The fusion construct termed BiPOLES is indicated by a black frame. (B) Representative photocurrents of βHK-Chrimson-mCerulean (top), Gt ACR1-ts-mCerulean-βHK-Chrimson (middle) Gt ACR2-ts-mCerulean-βHK-Chrimson (BiPOLES, bottom) in whole-cell patch clamp recordings from HEK293 cells at 490, 600 and 650 nm illumination. (C) Normalized peak photocurrents of BiPOLES at different membrane voltages evoked at either 490 or 600 nm (see panel B, mean ± SD; n = 6 - 8; normalized to the peak photocurrent at −80 mV and 600 nm illumination). (D) Reversal potential of early peak photocurrents during 500-ms illumination with 490, 600, or 650 nm light as shown in (B) (mean ± SD; n = 5 - 8). (E) Top: Representative photocurrents of BiPOLES with 10 ms light pulses of different color and equal photon flux at −60 mV. Lower left: Action spectra of BiPOLES at different membrane voltages (λ rev = photocurrent reversal wavelength, mean ± SEM, n = 4 - 9). Lower right: λ rev of Gt ACR1-ts-mCerulean-βHK-Chrimson and BiPOLES at different membrane voltages (mean ± SD; n = 3 - 9). (F) Peak photocurrent densities at −80 mV and 600 nm or 650 nm illumination as shown in (B) (Mean ± SD; n = 5 - 8).

Article Snippet: For HEK-cell expression, the coding sequences of Chrimson (KF992060.1) and CsChrimson (KJ995863.2) from Chlamydomonas noctigama , iC++ (Addgene #98165) , Aurora (Addgene #98217) , Gt ACR1 (KP171708) and Gt ACR2 (KP171709) from Guillardia theta were cloned together with mCerulean3 and a trafficking signal (ts) from the Kir 2.1 channel into a pCDNA3.1 vector containing the original opsin tandem cassette with a linker composed of eYFP and the first 105 N-terminal amino acids of the rat gastric H+/K+-ATPase beta subunit (βHK, NM_012510.2), kindly provided by Sonja Kleinlogel (University of Bern, CH).

Techniques: Construct, Patch Clamp